G-6-PD | GAL | PKU | MSUD

PKU

Punch a 4.75mm DBS disc into a "U" botton microplate, adding 100 microlitros of TCA 3% in water.

 

Incubate 30 minutes ar RT.

 

Transfer 40 microliters of eluate to a flat botton microplate.

 

Add 100 microliters of work enzyme solution ( 1 part of Enzyme + 3 parts of Co-enzyme).

Incubate 30 minutes at RT.

Add 100 microliters of Color Reagent and incubate for 2 to 5 minutes.

 

 

Read absorbance at 570 nm